SM0098 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Altritol nucleic acid* 2-Deoxy 2-O-Methyl* 2-Cyanoethyl
siRNA modification types 2 2
Overall number of modifications 6 2
Position of modifications 5,8,11,14,17 * 22 1 * 18
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
OCC12COC(CO1)C2O
OCC1OCCC1O


COC1C(O)OC(CO)C1O
OCC1OCC(OCC#N)C1O


siRNA Sequence GACGUAAACGGCCACAAGUUCT ACUUGUGGCCGUUUACGUCGCU
siRNA length base-pair 22 22
siRNA name in paper GS2370 DO1002
Biological activity 6.7 percent target mRNA inhibition
Experiment used to check activity Luciferase reporter assay
Melting temperature (oC) NA
Target gene EGFP gene
siRNA concentration 10 nM
Cell or Organism used HeLa cells
Transfection method Interfer (Polypus -Transfection)
Duration after transfection 72 Hours
Article title A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity
Reference19282453