Field | Sense | Antisense |
siRNA chemical modification | 2-Deoxy* Locked nucleic acid | 2-O-Methyl* 2-Cyanoethyl |
siRNA modification types | 2 | 2 |
Overall number of modifications | 22 | 2 |
Position of modifications | 1,2,4,5,7,8,10,11,13,14,16,17,19,22 * 3,6,9,12,15,18,20,21 | 1 * 18 |
Modification on sugar or base or phosphate | Sugar | Sugar |
SMILES (Click to view structure & nomenclature) |
OCC1OCCC1O OCC12COC(CO1)C2O |
COC1C(O)OC(CO)C1O OCC1OCC(OCC#N)C1O |
siRNA Sequence | GACGTAAACGGCCACAAGTTCT | ACUUGUGGCCGUUUACGUCGCU |
siRNA length base-pair | 22 | 22 |
siRNA name in paper | W008 | DO1002 |
Biological activity | 6.9 percent target mRNA inhibition | |
Experiment used to check activity | Luciferase reporter assay | |
Melting temperature (oC) | NA | |
Target gene | EGFP gene | |
siRNA concentration | 10 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Interfer (Polypus -Transfection) | |
Duration after transfection | 72 Hours | |
Article title | A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity |
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Reference | 19282453 |