| Field | Sense | Antisense |
| siRNA chemical modification | 2-N-Adamant-1-yl-Methylcarbonyl-2-Amino-Locked nucleic acid* Locked nucleic acid | Altritol nucleic acid |
| siRNA modification types | 2 | 1 |
| Overall number of modifications | 3 | 6 |
| Position of modifications | 5,20 * 21 | 3,6,14,18,20,21 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC12CN(C(CO1)C2O)C(=O)CC12CC3CC(CC(C3)C1)C2 OCC12COC(CO1)C2O |
OCC12COC(CO1)C2O |
| siRNA Sequence | GACGTAAACGGCCACAAGUTCU | ACUUGUGGCCGUUUACGUCGCU |
| siRNA length base-pair | 22 | 22 |
| siRNA name in paper | W069 | GS2378 |
| Biological activity | 70.2 percent target mRNA inhibition | |
| Experiment used to check activity | Luciferase reporter assay | |
| Melting temperature (oC) | NA | |
| Target gene | EGFP gene | |
| siRNA concentration | 10 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Interfer (Polypus -Transfection) | |
| Duration after transfection | 72 Hours | |
| Article title | A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity |
|
| Reference | 19282453 | |