| Field | Sense | Antisense |
| siRNA chemical modification | 2-Deoxy* Locked nucleic acid | Hexitol nucleic acid* 2-Deoxy |
| siRNA modification types | 2 | 2 |
| Overall number of modifications | 21 | 4 |
| Position of modifications | 1,3,5,7,8,9,11,13,15,17,19,22 * 2,4,6,10,12,14,16,18,20,21 | 18,19,20 * 21 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC1OCCC1O OCC12COC(CO1)C2O |
OCC1OCCCC1O OCC1OCCC1O |
| siRNA Sequence | GACGTAAACGGCCACAAGTTCT | ACUUGUGGCCGUUUACGUCGT |
| siRNA length base-pair | 22 | 21 |
| siRNA name in paper | W009 | GS2537 |
| Biological activity | 0 percent target mRNA inhibition | |
| Experiment used to check activity | Luciferase reporter assay | |
| Melting temperature (oC) | NA | |
| Target gene | EGFP gene | |
| siRNA concentration | 10 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Interfer (Polypus -Transfection) | |
| Duration after transfection | 72 Hours | |
| Article title | A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity |
|
| Reference | 19282453 | |