| Field | Sense | Antisense |
| siRNA chemical modification | UnLocked nucleic acid* Locked nucleic acid | Hexitol nucleic acid* 2-Deoxy |
| siRNA modification types | 2 | 2 |
| Overall number of modifications | 3 | 5 |
| Position of modifications | 10 * 21,22 | 2,18,19,20 * 21 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCCOC(CO)CO OCC12COC(CO1)C2O |
OCC1OCCCC1O OCC1OCCC1O |
| siRNA Sequence | GACGUAAACCGGCCACAAGUTCU | ACUUGUGGCCGUUUACGUCGT |
| siRNA length base-pair | 23 | 21 |
| siRNA name in paper | W130 | GS2538 |
| Biological activity | 0 percent target mRNA inhibition | |
| Experiment used to check activity | Luciferase reporter assay | |
| Melting temperature (oC) | NA | |
| Target gene | EGFP gene | |
| siRNA concentration | 10 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Interfer (Polypus -Transfection) | |
| Duration after transfection | 72 Hours | |
| Article title | A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity |
|
| Reference | 19282453 | |