SM1411 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Aminoethoxymethyl* 2-Deoxy 0
siRNA modification types 2 0
Overall number of modifications 6 0
Position of modifications 3,5,9,19,20 * 21 0
Modification on sugar or base or phosphate Sugar 0
SMILES
(Click to view structure & nomenclature)
NCCOCOC1COC(CO)C1O
OCC1OCCC1O


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siRNA Sequence GACGUAAACGGCCACAAGUUT ACUUGUGGCCGUUUACGUCGC
siRNA length base-pair 21 21
siRNA name in paper GS2543 W053
Biological activity 89.3 percent target mRNA inhibition
Experiment used to check activity Luciferase reporter assay
Melting temperature (oC) NA
Target gene EGFP gene
siRNA concentration 10 nM
Cell or Organism used HeLa cells
Transfection method Interfer (Polypus -Transfection)
Duration after transfection 72 Hours
Article title A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity
Reference19282453