Field | Sense | Antisense |
siRNA chemical modification | Locked nucleic acid | 2-N-Pyren-1-yl Methyl-2-Amino-Locked nucleic acid* Locked nucleic acid |
siRNA modification types | 1 | 2 |
Overall number of modifications | 3 | 3 |
Position of modifications | 5,20,21 | 6 * 20,21 |
Modification on sugar or base or phosphate | Sugar | Sugar |
SMILES (Click to view structure & nomenclature) |
OCC12COC(CO1)C2O |
OCC12CN(CC3=CC=C4C=CC5=CC=CC6=CC=C3C4=C56)C(CO1)C2O OCC12COC(CO1)C2O |
siRNA Sequence | GACGTAAACGGCCACAAGUTCU | ACUUGTGGCCGUUUACGUCGCU |
siRNA length base-pair | 22 | 22 |
siRNA name in paper | W011 | W096 |
Biological activity | 60 percent target mRNA inhibition | |
Experiment used to check activity | Luciferase reporter assay | |
Melting temperature (oC) | NA | |
Target gene | EGFP gene | |
siRNA concentration | 10 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Interfer (Polypus -Transfection) | |
Duration after transfection | 72 Hours | |
Article title | A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity |
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Reference | 19282453 |