SM2164 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-O-Methyl-4-Thioribose* 2-Deoxy 2-O-Methyl-4-Thioribose* 2-Deoxy
siRNA modification types 2 2
Overall number of modifications 10 6
Position of modifications 1,2,3,4,16,17,18,19 * 20,21 16,17,18,19 * 20,21
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
COC1CSC(CO)C1O
OCC1OCCC1O


COC1CSC(CO)C1O
OCC1OCCC1O


siRNA Sequence GCGCUGCUGGUGCCAACCCTT GGGUUGGCACCAGCAGCGCTT
siRNA length base-pair 21 21
siRNA name in paper SM1 SM1
Biological activity 48 percent target mRNA inhibition
Experiment used to check activity QRT PCR of luciferase.
Melting temperature (oC) Inreased (6.9)
Target gene Luciferase gene
siRNA concentration 120 nM
Cell or Organism used HeLa cells
Transfection method Envelop type Nano Device (MEND)
Duration after transfection NA
Article title Intracellular stability of 2'-OMe-4'-thioribonucleoside modified siRNA leads to long-term RNAi effect
Reference22411910