| Field | Sense | Antisense |
| siRNA chemical modification | 2-O-Methyl-4-Thioribose* 2-Deoxy | 2-Deoxy |
| siRNA modification types | 2 | 1 |
| Overall number of modifications | 8 | 2 |
| Position of modifications | 1,2,3,4,18,19 * 20,21 | 20,21 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
COC1CSC(CO)C1O OCC1OCCC1O |
OCC1OCCC1O |
| siRNA Sequence | GCGCUGCUGGUGCCAACCCTT | GGGUUGGCACCAGCAGCGCTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | SM4 | SM4 |
| Biological activity | 66 percent target mRNA inhibition | |
| Experiment used to check activity | QRT PCR of luciferase. | |
| Melting temperature (oC) | Inreased (3.0) | |
| Target gene | Luciferase gene | |
| siRNA concentration | 120 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Envelop type Nano Device (MEND) | |
| Duration after transfection | NA | |
| Article title | Intracellular stability of 2'-OMe-4'-thioribonucleoside modified siRNA leads to long-term RNAi effect |
|
| Reference | 22411910 | |