| Field | Sense | Antisense |
| siRNA chemical modification | 0 | Methylcytosine* Propynyluridine |
| siRNA modification types | 0 | 2 |
| Overall number of modifications | 0 | 10 |
| Position of modifications | 0 | 6,8,9,12,15 * 2,4,11,14,19 |
| Modification on sugar or base or phosphate | 0 | Base |
| SMILES (Click to view structure & nomenclature) |
- |
CC1=C(N)NC(=O)N=C1 CC#CC1=CNC(=O)NC1=O |
| siRNA Sequence | AUCUGAAGAAGGAGAAAAATT | UUUUUCUCCUUCUUCAGAUTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | ss (passenger) 5 | 5 |
| Biological activity | 82 percent target mRNA inhibition | |
| Experiment used to check activity | Renilla luciferase mRNA suppression | |
| Melting temperature (oC) | 82 | |
| Target gene | Luciferase gene | |
| siRNA concentration | 0.3 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 22 Hours | |
| Article title | RNA major groove modifications improve siRNA stability and biological activity |
|
| Reference | 19042976 | |