SM2177 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 Propynyluridine
siRNA modification types 0 1
Overall number of modifications 0 6
Position of modifications 0 1,2,3,4,5,7
Modification on sugar or base or phosphate 0 Base
SMILES
(Click to view structure & nomenclature)
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CC#CC1=CNC(=O)NC1=O



siRNA Sequence AUCUGAAGAAGGAGAAAAATT UUUUUCUCCUUCUUCAGAUTT
siRNA length base-pair 21 21
siRNA name in paper ss (passenger) 6 6
Biological activity 41 percent target mRNA inhibition
Experiment used to check activity Renilla luciferase mRNA suppression
Melting temperature (oC) 77.8
Target gene Luciferase gene
siRNA concentration 0.3 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 22 Hours
Article title RNA major groove modifications improve siRNA stability and biological activity
Reference19042976