Field | Sense | Antisense |
siRNA chemical modification | 2-O-Methyl* Phosphorothioate | 2-O-Methyl |
siRNA modification types | 2 | 1 |
Overall number of modifications | 9 | 5 |
Position of modifications | 8,9,12,14,15,17,18 * 19 | 14,15,17,18,19 |
Modification on sugar or base or phosphate | Sugar; Phosphate | Sugar |
SMILES (Click to view structure & nomenclature) |
COC1C(O)OC(CO)C1O OCC1OCC(O)C1OP(O)(S)=O |
COC1C(O)OC(CO)C1O |
siRNA Sequence | AAGGAGAUCAACAUUUUCATT | UGAAAAUGUUGAUCUCCUUTT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | Sur10058-Me-Py2 | Sur10058-Me-Py2 |
Biological activity | 84 percent target mRNA inhibition | |
Experiment used to check activity | Real-time PCR | |
Melting temperature (oC) | NA | |
Target gene | Survivin gene | |
siRNA concentration | 2 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 24 Hours | |
Article title | Chemical modification of siRNAs to improve serum stability without loss of efficacy |
|
Reference | 16598842 |