| Field | Sense | Antisense |
| siRNA chemical modification | 2-O-Methyl* Phosphorothioate | Phosphorothioate* 2-O-Methyl |
| siRNA modification types | 2 | 2 |
| Overall number of modifications | 14 | 17 |
| Position of modifications | 8,9,12,14,15,16,17,18 * 2,3,4,5,6,7 | 2,3,4,5,6,7,8,9,10,11,12,13 *14,15,17,18,19 |
| Modification on sugar or base or phosphate | Sugar; Phosphate | Phosphate; Sugar |
| SMILES (Click to view structure & nomenclature) |
COC1C(O)OC(CO)C1O OCC1OCC(O)C1OP(O)(S)=O |
OCC1OCC(O)C1OP(O)(S)=O COC1C(O)OC(CO)C1O |
| siRNA Sequence | AAGGAGAUCAACAUUUUCATT | UGAAAAUGUUGAUCUCCUUTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | Sur10058-Me-Py3 | Sur10058-Me-Py3 |
| Biological activity | 77 percent target mRNA inhibition | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | Survivin gene | |
| siRNA concentration | 2 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 24 Hours | |
| Article title | Chemical modification of siRNAs to improve serum stability without loss of efficacy |
|
| Reference | 16598842 | |