| Field | Sense | Antisense |
| siRNA chemical modification | 2-O-Methyl | 2-Fluoro* Inverted DeoxyThymidine |
| siRNA modification types | 1 | 2 |
| Overall number of modifications | 8 | 12 |
| Position of modifications | 8,9,12,14,15,16,17,18 | 1,7,9,10,13,14,15,16,17,18,19 *21 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
COC1C(O)OC(CO)C1O |
OCC1OCC(F)C1O CC1=CN(C2CC(OP([O-])(=O)OC3CCOC3CO)C(CO)O2)C(=O)NC1=O |
| siRNA Sequence | AAGGAGAUCAACAUUUUCATT | UGAAAAUGUUGAUCUCCUUTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | Sur10058-Me-Py6 | Sur10058-Me-Py6 |
| Biological activity | 77 percent target mRNA inhibition | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | Survivin gene | |
| siRNA concentration | 2 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 24 Hours | |
| Article title | Chemical modification of siRNAs to improve serum stability without loss of efficacy |
|
| Reference | 16598842 | |