SM2275 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 4-Thioribose 0
siRNA modification types 1 0
Overall number of modifications 4 0
Position of modifications 4,10,11,17 0
Modification on sugar or base or phosphate Sugar 0
SMILES
(Click to view structure & nomenclature)
OCC1SCC(O)C1O



-



siRNA Sequence AAGUAAGGACCAGAGACAA UUGUCUCUGGUCCUUACUU
siRNA length base-pair 19 19
siRNA name in paper siRNA-14 siRNA-14
Biological activity IC-50 = 1.1 nM for target mRNA
Experiment used to check activity mRNA analysis
Melting temperature (oC) NA
Target gene Human PTEN gene
siRNA concentration 1.1 nM
Cell or Organism used HeLa cells
Transfection method Lipofectin (Invitrogen)
Duration after transfection 41 Hours
Article title Improving RNA interference in mammalian cells by 4'-thio-modified small interfering RNA (siRNA): effect on siRNA activity and nuclease stability when used in combination with 2'-O-alkyl modifications
Reference16509579