| Field | Sense | Antisense |
| siRNA chemical modification | 2-O-(2-Methoxyethyl) | 0 |
| siRNA modification types | 1 | 0 |
| Overall number of modifications | 6 | 0 |
| Position of modifications | 1,2,3,17,18,19 | 0 |
| Modification on sugar or base or phosphate | Sugar | 0 |
| SMILES (Click to view structure & nomenclature) |
COCCOC1COC(CO)C1O |
- |
| siRNA Sequence | AAGUAAGGACCAGAGACAA | UUGUCUCUGGUCCUUACUU |
| siRNA length base-pair | 19 | 19 |
| siRNA name in paper | siRNA-20 | siRNA-20 |
| Biological activity | IC-50 = 0.58 nM for target mRNA | |
| Experiment used to check activity | mRNA analysis | |
| Melting temperature (oC) | 74.3 | |
| Target gene | Human PTEN gene | |
| siRNA concentration | 0.58 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectin (Invitrogen) | |
| Duration after transfection | 53 Hours | |
| Article title | Improving RNA interference in mammalian cells by 4'-thio-modified small interfering RNA (siRNA): effect on siRNA activity and nuclease stability when used in combination with 2'-O-alkyl modifications |
|
| Reference | 16509579 | |