| Field | Sense | Antisense |
| siRNA chemical modification | 4-Thioribose | 4-Thioribose* 2-O-Methyl |
| siRNA modification types | 1 | 2 |
| Overall number of modifications | 4 | 5 |
| Position of modifications | 1,2,18,19 | 1,2 * 17,18,19 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC1SCC(O)C1O |
OCC1SCC(O)C1O COC1C(O)OC(CO)C1O |
| siRNA Sequence | AAGUAAGGACCAGAGACAA | UUGUCUCUGGUCCUUACUU |
| siRNA length base-pair | 19 | 19 |
| siRNA name in paper | siRNA-25 | siRNA-25 |
| Biological activity | IC-50 = 0.15 nM for target mRNA | |
| Experiment used to check activity | mRNA analysis | |
| Melting temperature (oC) | 75.4 | |
| Target gene | Human PTEN gene | |
| siRNA concentration | 0.15 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectin (Invitrogen) | |
| Duration after transfection | 63 Hours | |
| Article title | Improving RNA interference in mammalian cells by 4'-thio-modified small interfering RNA (siRNA): effect on siRNA activity and nuclease stability when used in combination with 2'-O-alkyl modifications |
|
| Reference | 16509579 | |