| Field | Sense | Antisense |
| siRNA chemical modification | 2-Fluoro* 2-O-Methyl* 5-Phosphate | 2-O-Methyl* 2-Fluoro* 5-Phosphate |
| siRNA modification types | 3 | 3 |
| Overall number of modifications | 20 | 20 |
| Position of modifications | 1,3,5,7,9,11,13,15,17,19 * 2,4,6,8,10,12,14,16,18 * 1 | 1,3,5,7,9,11,13,15,17,19 * 2,4,6,8,10,12,14,16,18 * 1 |
| Modification on sugar or base or phosphate | Sugar; Sugar; Phosphate | Sugar; Sugar; Phosphate |
| SMILES (Click to view structure & nomenclature) |
OCC1OCC(F)C1O COC1C(O)OC(CO)C1O OC1COC(COP(O)([O-])=O)C1O |
COC1C(O)OC(CO)C1O OCC1OCC(F)C1O OC1COC(COP(O)([O-])=O)C1O |
| siRNA Sequence | GGGUAAAUACAUUCUUCAU | AUGAAGAAUGUAUUUACCC |
| siRNA length base-pair | 19 | 19 |
| siRNA name in paper | 11 | 11 |
| Biological activity | 83 percent target mRNA inhibition | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | 82 | |
| Target gene | Human PTEN gene | |
| siRNA concentration | 1 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectin (Invitrogen) | |
| Duration after transfection | 20 Hours | |
| Article title | Fully 2'-modified oligonucleotide duplexes with improved in vitro potency and stability compared to unmodified small interfering RNA |
|
| Reference | 15715458 | |