| Field | Sense | Antisense |
| siRNA chemical modification | Pseudouridine | 0 |
| siRNA modification types | 1 | 0 |
| Overall number of modifications | 1 | 0 |
| Position of modifications | 19 | 0 |
| Modification on sugar or base or phosphate | Base | 0 |
| SMILES (Click to view structure & nomenclature) |
OCC1OC(C(O)C1O)C1=CNC(=O)NC1=O |
- |
| siRNA Sequence | ACAUGAAGCAGCACGACUUTT | AAGUCGUGCUGCUUCAUGUTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | s&psi19 | aG2 |
| Biological activity | 70 percent target mRNA inhibition | |
| Experiment used to check activity | Dual fluorescence assay | |
| Melting temperature (oC) | 82.4 | |
| Target gene | EGFP gene | |
| siRNA concentration | 1 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 36 Hours | |
| Article title | Effect of base modifications on structure, thermodynamic stability, and gene silencing activity of short interfering RNA |
|
| Reference | 17585051 | |