Field | Sense | Antisense |
siRNA chemical modification | Dihydrouridine | 2-Thiouridine |
siRNA modification types | 1 | 1 |
Overall number of modifications | 1 | 1 |
Position of modifications | 19 | 19 |
Modification on sugar or base or phosphate | Base | Base |
SMILES (Click to view structure & nomenclature) |
O=C1CCNC(=O)N1 |
OCC1OC(C(O)C1O)N1C=CC(=O)NC1=S |
siRNA Sequence | ACAUGAAGCAGCACGACUUTT | AAGUCGUGCUGCUUCAUGUTT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | sD19 | as2U19 |
Biological activity | 92 percent target mRNA inhibition | |
Experiment used to check activity | Dual fluorescence assay | |
Melting temperature (oC) | 80.4 | |
Target gene | EGFP gene | |
siRNA concentration | 1 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 36 Hours | |
Article title | Effect of base modifications on structure, thermodynamic stability, and gene silencing activity of short interfering RNA |
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Reference | 17585051 |