SM2312 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Dihydrouridine Pseudouridine
siRNA modification types 1 1
Overall number of modifications 1 1
Position of modifications 19 10
Modification on sugar or base or phosphate Base Base
SMILES
(Click to view structure & nomenclature)
O=C1CCNC(=O)N1



OCC1OC(C(O)C1O)C1=CNC(=O)NC1=O



siRNA Sequence ACAUGAAGCAGCACGACUUTT AAGUCGUGCUGCUUCAUGUTT
siRNA length base-pair 21 21
siRNA name in paper sD19 aψ10
Biological activity 89 percent target mRNA inhibition
Experiment used to check activity Dual fluorescence assay
Melting temperature (oC) 81.4
Target gene EGFP gene
siRNA concentration 1 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 36 Hours
Article title Effect of base modifications on structure, thermodynamic stability, and gene silencing activity of short interfering RNA
Reference17585051