SM2322 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 Wobble Base Pair* 2-Thiouridine
siRNA modification types 0 2
Overall number of modifications 0 2
Position of modifications 0 8 * 9
Modification on sugar or base or phosphate 0 Base
SMILES
(Click to view structure & nomenclature)
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OCC1OC(C(O)C1O)N1C=CC(=O)NC1=S


siRNA Sequence AGCUUUGUGGAGAUGGUGGTT CCACCAUCUCCACAAAGCUTT
siRNA length base-pair 21 21
siRNA name in paper sB aW8s2U9
Biological activity 67 percent target mRNA inhibition
Experiment used to check activity Dual fluorescence assay
Melting temperature (oC) NA
Target gene EGFP gene
siRNA concentration 5 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 36 Hours
Article title Effect of base modifications on structure, thermodynamic stability, and gene silencing activity of short interfering RNA
Reference17585051