Field | Sense | Antisense |
siRNA chemical modification | Dihydrouridine | 0 |
siRNA modification types | 1 | 0 |
Overall number of modifications | 1 | 0 |
Position of modifications | 20 | 0 |
Modification on sugar or base or phosphate | Base | 0 |
SMILES (Click to view structure & nomenclature) |
O=C1CCNC(=O)N1 |
- |
siRNA Sequence | AUACAGGCAGCAGUAACUUUTT | AAAGUUACUGCUGCCUGUAUTT |
siRNA length base-pair | 22 | 22 |
siRNA name in paper | sD19 | aSYM |
Biological activity | 71 percent target mRNA inhibition | |
Experiment used to check activity | Dual fluorescence assay | |
Melting temperature (oC) | NA | |
Target gene | EGFP gene | |
siRNA concentration | 1 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 36 Hours | |
Article title | Effect of base modifications on structure, thermodynamic stability, and gene silencing activity of short interfering RNA |
|
Reference | 17585051 |