| Field | Sense | Antisense |
| siRNA chemical modification | Pseudouridine* Dihydrouridine | Pseudouridine |
| siRNA modification types | 2 | 1 |
| Overall number of modifications | 2 | 1 |
| Position of modifications | 2 * 20 | 20 |
| Modification on sugar or base or phosphate | Base | Base |
| SMILES (Click to view structure & nomenclature) |
OCC1OC(C(O)C1O)C1=CNC(=O)NC1=O O=C1CCNC(=O)N1 |
OCC1OC(C(O)C1O)C1=CNC(=O)NC1=O |
| siRNA Sequence | AUACAGGCAGCAGUAACUUUTT | AAAGUUACUGCUGCCUGUAUTT |
| siRNA length base-pair | 22 | 22 |
| siRNA name in paper | ss2U2,D19 | as2U19 |
| Biological activity | 77 percent target mRNA inhibition | |
| Experiment used to check activity | Dual fluorescence assay | |
| Melting temperature (oC) | NA | |
| Target gene | EGFP gene | |
| siRNA concentration | 1 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 36 Hours | |
| Article title | Effect of base modifications on structure, thermodynamic stability, and gene silencing activity of short interfering RNA |
|
| Reference | 17585051 | |