| Field | Sense | Antisense |
| siRNA chemical modification | Aminoisonucleotide-Adenine* 2-Deoxythymidine | 2-Deoxythymidine |
| siRNA modification types | 2 | 1 |
| Overall number of modifications | 3 | 2 |
| Position of modifications | 8* 20,21 | 20,21 |
| Modification on sugar or base or phosphate | Base | Sugar |
| SMILES (Click to view structure & nomenclature) |
NC1OC(CO)C(O)C1N1C=NC2=C(N)N=CN=C12 CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
OCC1OCCC1O |
| siRNA Sequence | CUGGUAAAGCAUUCAGUAUTT | AUACUGAAUGCUUUACCAGTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | ssIso-3 | asNC |
| Biological activity | 78 percent target mRNA inhibition | |
| Experiment used to check activity | Dual luciferase reporter assay | |
| Melting temperature (oC) | 62 | |
| Target gene | Luciferase gene | |
| siRNA concentration | 13 nM | |
| Cell or Organism used | HEK293 cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 31 Hours | |
| Article title | Studies on aminoisonucleoside modified siRNAs: stability and silencing activity |
|
| Reference | 17539595 | |