SM2340 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Deoxythymidine Aminoisonucleotide-Adenine* 2-Deoxythymidine
siRNA modification types 1 2
Overall number of modifications 2 3
Position of modifications 20,21 8* 20,21
Modification on sugar or base or phosphate Sugar Base; Sugar
SMILES
(Click to view structure & nomenclature) 		CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O
NC1OC(CO)C(O)C1N1C=NC2=C(N)N=CN=C12
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O
siRNA Sequence CUGGUAAAGCAUUCAGUAUTT AUACUGAAUGCUUUACCAGTT
siRNA length base-pair 21 21
siRNA name in paper ssNC asIso-3
Biological activity 2 percent target mRNA inhibition
Experiment used to check activity Dual luciferase reporter assay
Melting temperature (oC) NA
Target gene Luciferase gene
siRNA concentration 13 nM
Cell or Organism used HEK293 cells
Transfection method Lipofectamine 2000
Duration after transfection 41 Hours
Article title Studies on aminoisonucleoside modified siRNAs: stability and silencing activity
Reference17539595