| Field | Sense | Antisense |
| siRNA chemical modification | 2-Deoxythymidine | Aminoisonucleotide-thymidine* Aminoisonucleotide-adenine* 2-Deoxythymidine |
| siRNA modification types | 1 | 3 |
| Overall number of modifications | 2 | 3 |
| Position of modifications | 20,21 | 1* 20* 21 |
| Modification on sugar or base or phosphate | Sugar | Base; Sugar |
| SMILES (Click to view structure & nomenclature) |
CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
CC1=CN(C2C(N)OC(CO)C2O)C(=O)NC1=O NC1OC(CO)C(O)C1N1C=NC2=C(N)N=CN=C12 CC1=CN(C2CC(O)C(CO)O2)C(=O)NC1=O |
| siRNA Sequence | CUGGUAAAGCAUUCAGUAUTT | AUACUGAAUGCUUUACCAGTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | ssNC | asIso-4 |
| Biological activity | 13 percent target mRNA inhibition | |
| Experiment used to check activity | Dual luciferase reporter assay | |
| Melting temperature (oC) | NA | |
| Target gene | Luciferase gene | |
| siRNA concentration | 13 nM | |
| Cell or Organism used | HEK293 cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 43 Hours | |
| Article title | Studies on aminoisonucleoside modified siRNAs: stability and silencing activity |
|
| Reference | 17539595 | |