SM2346 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 12-[(2,3,4,6-Tetra-O-methyl-b-D-glucopyranosyloxy)]dodecyl (2-cyanoethyl) (N,N-diisopropyl) phosphoramidite derivative 0
siRNA modification types 1 0
Overall number of modifications 1 0
Position of modifications 1 0
Modification on sugar or base or phosphate Phosphate 0
SMILES
(Click to view structure & nomenclature)
COC1OC(OCCCCCCCCCCCCOP(O)(=O)OCCCCC(=O)NCC(CNC(=O)CCCCOP(O)(=O)OCCCCCCCCCCCCOC2OC(OC)C(OC)C(OC)C2OC)OP(O)(O)=O)C(OC)C(OC)C1OC



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siRNA Sequence AUCUGAAGAAGGAGAAAAATT UUUUUCUCCUUCUUCAGAUTT
siRNA length base-pair 21 21
siRNA name in paper 3 3
Biological activity 79 percent target mRNA inhibition
Experiment used to check activity Luciferase reporter assay
Melting temperature (oC) NA
Target gene Luciferase gene
siRNA concentration 0.8 nM
Cell or Organism used HeLa and HuH-cells
Transfection method Lipofectamine 2007
Duration after transfection 24 Hours
Article title Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
Reference23764303