SM2376 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Glucosamine analogue Glucosamine analogue
siRNA modification types 1 1
Overall number of modifications 1 1
Position of modifications 22 22
Modification on sugar or base or phosphate Base Base
SMILES
(Click to view structure & nomenclature)
NC1C(O)C(O)C(CO)OC1OCCOP(O)([O-])=O



NC1C(O)C(O)C(CO)OC1OCCOP(O)([O-])=O



siRNA Sequence CUUCUUCGUCGAGACCAUGTTY CAUGGUCUCGACGAAGAAGTTY
siRNA length base-pair 22 22
siRNA name in paper siRNA 27 siRNA 27
Biological activity 22 percent target mRNA inhibition
Experiment used to check activity Dual luciferase reporter assay
Melting temperature (oC) 74
Target gene Luciferase gene
siRNA concentration 0.1 nM
Cell or Organism used HeLa cells
Transfection method TransFast (Promega)
Duration after transfection 24 Hours
Article title Synthesis of oligonucleotides with glucosamine at the 3'-position and evaluation of their biological activity
Reference23743279