Field | Sense | Antisense |
siRNA chemical modification | Thymidine analogue | Thymidine analogue |
siRNA modification types | 1 | 1 |
Overall number of modifications | 1 | 1 |
Position of modifications | 20 | 20 |
Modification on sugar or base or phosphate | Base | Base |
SMILES (Click to view structure & nomenclature) |
CC1=CN(CC2OC(OCCOP(O)([O-])=O)C(N)C(O)C2O)C(=O)NC1=O |
CC1=CN(CC2OC(OCCOP(O)([O-])=O)C(N)C(O)C2O)C(=O)NC1=O |
siRNA Sequence | CUUCUUCGUCGAGACCAUGX | CAUGGUCUCGACGAAGAAGX |
siRNA length base-pair | 20 | 20 |
siRNA name in paper | siRNA 22 | siRNA 22 |
Biological activity | 42 percent target mRNA inhibition | |
Experiment used to check activity | Dual luciferase reporter assay | |
Melting temperature (oC) | 75.4 | |
Target gene | Luciferase gene | |
siRNA concentration | 1 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | TransFast (Promega) | |
Duration after transfection | 24 Hours | |
Article title | Synthesis of oligonucleotides with glucosamine at the 3'-position and evaluation of their biological activity |
|
Reference | 23743279 |