| Field | Sense | Antisense |
| siRNA chemical modification | Glucosamine analogue | Glucosamine analogue |
| siRNA modification types | 1 | 1 |
| Overall number of modifications | 1 | 1 |
| Position of modifications | 21 | 21 |
| Modification on sugar or base or phosphate | Base | Base |
| SMILES (Click to view structure & nomenclature) |
NC1C(O)C(O)C(CO)OC1OCCOP(O)([O-])=O |
NC1C(O)C(O)C(CO)OC1OCCOP(O)([O-])=O |
| siRNA Sequence | CUUCUUCGUCGAGACCAUGTY | CAUGGUCUCGACGAAGAAGTY |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | siRNA 25 | siRNA 25 |
| Biological activity | 53 percent target mRNA inhibition | |
| Experiment used to check activity | Dual luciferase reporter assay | |
| Melting temperature (oC) | 75.1 | |
| Target gene | Luciferase gene | |
| siRNA concentration | 1 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | TransFast (Promega) | |
| Duration after transfection | 24 Hours | |
| Article title | Synthesis of oligonucleotides with glucosamine at the 3'-position and evaluation of their biological activity |
|
| Reference | 23743279 | |