SM2388 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Glucosamine analogue Glucosamine analogue
siRNA modification types 1 1
Overall number of modifications 1 1
Position of modifications 21 21
Modification on sugar or base or phosphate Base Base
SMILES
(Click to view structure & nomenclature)
NC1C(O)C(O)C(CO)OC1OCCOP(O)([O-])=O



NC1C(O)C(O)C(CO)OC1OCCOP(O)([O-])=O



siRNA Sequence CUUCUUCGUCGAGACCAUGTY CAUGGUCUCGACGAAGAAGTY
siRNA length base-pair 21 21
siRNA name in paper siRNA 25 siRNA 25
Biological activity 68 percent target mRNA inhibition
Experiment used to check activity Dual luciferase reporter assay
Melting temperature (oC) 75.1
Target gene Luciferase gene
siRNA concentration 10 nM
Cell or Organism used HeLa cells
Transfection method TransFast (Promega)
Duration after transfection 24 Hours
Article title Synthesis of oligonucleotides with glucosamine at the 3'-position and evaluation of their biological activity
Reference23743279