SM2415 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Boron cluster Boron cluster
siRNA modification types 1 1
Overall number of modifications 1 1
Position of modifications 3 20
Modification on sugar or base or phosphate Base Base
SMILES
(Click to view structure & nomenclature) 		[B]1234[B]567[B]118[B]229[B]11%10[B]22%11[B]%12%13%14[B]35([B]6%123[B]12%13[C]78%103)[C]49%11%14
[B]1234[B]567[B]118[B]229[B]11%10[B]22%11[B]%12%13%14[B]35([B]6%123[B]12%13[C]78%103)[C]49%11%14
siRNA Sequence AAUCAGACAAGUUCUUCAUTT AUGAAGAACUUGUCUGAUUTT
siRNA length base-pair 21 21
siRNA name in paper (CB3)s (CB3)as
Biological activity 97 percent target mRNA inhibition
Experiment used to check activity Dual fluorescence assay
Melting temperature (oC) 64.1 ±0.2
Target gene BACE1 gene
siRNA concentration 1 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 24 Hours
Article title siRNAs modified with boron cluster and their physicochemical and biological characterization
Reference23682800