SM2420 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Boron cluster Boron cluster
siRNA modification types 1 1
Overall number of modifications 1 1
Position of modifications 20 20
Modification on sugar or base or phosphate Base Base
SMILES
(Click to view structure & nomenclature) 		[B]1234[B]567[B]118[B]229[B]11%10[B]22%11[B]%12%13%14[B]35([B]6%123[B]12%13[C]78%103)[C]49%11%14
[B]1234[B]567[B]118[B]229[B]11%10[B]22%11[B]%12%13%14[B]35([B]6%123[B]12%13[C]78%103)[C]49%11%14
siRNA Sequence AAUCAGACAAGUUCUUCAUTT AUGAAGAACUUGUCUGAUUTT
siRNA length base-pair 21 21
siRNA name in paper (CB20)s (CB20)as
Biological activity 58 percent target mRNA inhibition
Experiment used to check activity Dual fluorescence assay
Melting temperature (oC) 61.6 ±0.2
Target gene BACE1 gene
siRNA concentration 0.1 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 24 Hours
Article title siRNAs modified with boron cluster and their physicochemical and biological characterization
Reference23682800