SM2423 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Deoxyuridine Deoxyadenine
siRNA modification types 1 1
Overall number of modifications 5 5
Position of modifications 21,22,23,24,25 21,22,23,24,25
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
OCC1OC(CC1O)N1C=CC(=O)NC1=O



NC1=NC=NC2=C1N=CN2C1CC(O)C(CO)O1



siRNA Sequence GGAUAUUGUCAGUCUUUAGGUUUUU CCUAAAGACUGACAAUAUCCAAAAA
siRNA length base-pair 25 25
siRNA name in paper T S6-dU/dA T S6-dU/dA
Biological activity 62 percent target mRNA inhibition
Experiment used to check activity Real-time quantitative PCR
Melting temperature (oC) 47.5 ± 0.9
Target gene Thymidylate synthase gene
siRNA concentration 1 nM
Cell or Organism used RKO cells
Transfection method Lipofectamine 2000
Duration after transfection 24 Hours
Article title Development of modified siRNA molecules incorporating 5-fluoro-2'-deoxyuridine residues to enhance cytotoxicity
Reference23449220