| Field | Sense | Antisense |
| siRNA chemical modification | Deoxyuridine | 5-Fluoro-2-Deoxyuridine* Deoxyadenine |
| siRNA modification types | 1 | 2 |
| Overall number of modifications | 5 | 7 |
| Position of modifications | 21,22,23,24,25 | 16,18 * 21,22,23,24,25 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC1OC(CC1O)N1C=CC(=O)NC1=O |
OCC1OC(CC1O)N1C=C(F)C(=O)NC1=O NC1=NC=NC2=C1N=CN2C1CC(O)C(CO)O1 |
| siRNA Sequence | GGAUAUUGUCAGUCUUUAGGUUUUU | CCUAAAGACUGACAAUAUCCAAAAA |
| siRNA length base-pair | 25 | 25 |
| siRNA name in paper | TS6-2xF-dU/dA | TS6-2xF-dU/dA |
| Biological activity | 25 percent target mRNA inhibition | |
| Experiment used to check activity | Real-time quantitative PCR | |
| Melting temperature (oC) | 54.7 ± 1.6 | |
| Target gene | Thymidylate synthase gene | |
| siRNA concentration | 1 nM | |
| Cell or Organism used | RKO cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 24 Hours | |
| Article title | Development of modified siRNA molecules incorporating 5-fluoro-2'-deoxyuridine residues to enhance cytotoxicity |
|
| Reference | 23449220 | |