SM2434 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 5-Fluoro-2-Deoxyuridine* Mismatch 5-Fluoro-2-Deoxyuridine* Mismatch
siRNA modification types 2 2
Overall number of modifications 9 9
Position of modifications 21,22,23,24,25 * 6,9,12,16 21,22,23,24,25 * 5,9,12,15
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature) 		OCC1OC(CC1O)N1C=C(F)C(=O)NC1=O
OCC1OC(CC1O)N1C=C(F)C(=O)NC1=O
siRNA Sequence GGAUACUGCCAAUCUCUAGGUUUUU CCUAGAGAUUGGCAGUAUCCAAAAA
siRNA length base-pair 25 25
siRNA name in paper Mis-FdU/dA Mis-FdU/dA
Biological activity 0 percent target mRNA inhibition
Experiment used to check activity Real-time quantitative PCR
Melting temperature (oC) NA
Target gene Thymidylate synthase gene
siRNA concentration 1 nM
Cell or Organism used RKO cells
Transfection method Lipofectamine 2000
Duration after transfection 24 Hours
Article title Development of modified siRNA molecules incorporating 5-fluoro-2'-deoxyuridine residues to enhance cytotoxicity
Reference23449220