SM2456 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-O-Methyl* Inverted abasic 2-O-Methyl* 2-O-Benzyl
siRNA modification types 2 2
Overall number of modifications 4 3
Position of modifications 20,21 * 1,21 20,21 * 5
Modification on sugar or base or phosphate Sugar; Base Sugar
SMILES
(Click to view structure & nomenclature)
COC1C(O)OC(CO)C1O
CC1COC(CO)C1OP(O)([O-])=O


COC1C(O)OC(CO)C1O
OCC1OCC(OC2=CC=CC=C2)C1O


siRNA Sequence CAAGUGUCAUCACACUGAAUU UUCAGUGUGAUGACACUUGUU
siRNA length base-pair 21 21
siRNA name in paper ApoB(10162)-5 ApoB(10162)-5
Biological activity 94 percent target mRNA inhibition
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene Apolipoprotein B gene
siRNA concentration 3 mg/kg
Cell or Organism used C57BL/6 mice
Transfection method Lipid and siRNA injected
Duration after transfection 72 Hours (3 days)
Article title siRNA-optimized Modifications for Enhanced In Vivo Activity
Reference23344622