| Field | Sense | Antisense |
| siRNA chemical modification | 2-O-Methyl* Inverted abasic | 2-O-Methyl* 2-O-Benzyl |
| siRNA modification types | 2 | 2 |
| Overall number of modifications | 4 | 3 |
| Position of modifications | 20,21 * 1,21 | 20,21 * 5 |
| Modification on sugar or base or phosphate | Sugar; Base | Sugar |
| SMILES (Click to view structure & nomenclature) |
COC1C(O)OC(CO)C1O CC1COC(CO)C1OP(O)([O-])=O |
COC1C(O)OC(CO)C1O OCC1OCC(OC2=CC=CC=C2)C1O |
| siRNA Sequence | CAAGUGUCAUCACACUGAAUU | UUCAGUGUGAUGACACUUGUU |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | ApoB(10162)-5 | ApoB(10162)-5 |
| Biological activity | 94 percent target mRNA inhibition | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | Apolipoprotein B gene | |
| siRNA concentration | 3 mg/kg | |
| Cell or Organism used | C57BL/6 mice | |
| Transfection method | Lipid and siRNA injected | |
| Duration after transfection | 72 Hours (3 days) | |
| Article title | siRNA-optimized Modifications for Enhanced In Vivo Activity |
|
| Reference | 23344622 | |