SM2465 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Assymetric siRNA (3-End Overhang) Assymetric siRNA (3-End Overhang)
siRNA modification types 1 1
Overall number of modifications 1 1
Position of modifications 21 1
Modification on sugar or base or phosphate NA NA
SMILES
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siRNA Sequence CGGAGGCUGGGAUGCCUUUTT AAAGGCAUCCCAGCCUCCGTT
siRNA length base-pair 21 21
siRNA name in paper siRNA(19bp) siRNA(19bp)
Biological activity 60 percent target mRNA inhibition
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene Bcl-2 gene
siRNA concentration 33 nM
Cell or Organism used HeLa B2 cells
Transfection method Lipofectamine 2000
Duration after transfection 48 Hours
Article title Asymmetric siRNA targeting the bcl 2 gene inhibits the proliferation of cancer cells in vitro and in vivo
Reference23135004