Field | Sense | Antisense |
siRNA chemical modification | Assymetric siRNA (3-End Overhang) | Assymetric siRNA (3-End Overhang) |
siRNA modification types | 1 | 1 |
Overall number of modifications | 1 | 1 |
Position of modifications | 18 | 1 |
Modification on sugar or base or phosphate | NA | NA |
SMILES (Click to view structure & nomenclature) |
- |
- |
siRNA Sequence | AGGCUGGGAUGCCUUUTT | AAAGGCAUCCCAGCCUCCGTT |
siRNA length base-pair | 18 | 21 |
siRNA name in paper | siRNA(16bp) | siRNA(16bp) |
Biological activity | 18 percent target mRNA inhibition | |
Experiment used to check activity | Real-time PCR | |
Melting temperature (oC) | NA | |
Target gene | Bcl-2 gene | |
siRNA concentration | 33 nM | |
Cell or Organism used | HeLa B2 cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 48 Hours | |
Article title | Asymmetric siRNA targeting the bcl 2 gene inhibits the proliferation of cancer cells in vitro and in vivo | |
Reference | 23135004 |