| Field | Sense | Antisense |
| siRNA chemical modification | Cholesterol* Fluorouridine* Phosphorothioate | 0 |
| siRNA modification types | 3 | 0 |
| Overall number of modifications | 11 | 0 |
| Position of modifications | 1 * 2,3,4,14,15,16,17 * 15,16,17 | 0 |
| Modification on sugar or base or phosphate | Phosphate; Base; Phosphate | 0 |
| SMILES (Click to view structure & nomenclature) |
CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C OCC1OC(C(O)C1O)N1C=C(F)C(=O)NC1=O OCC1OCC(O)C1OP(O)(S)=O |
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| siRNA Sequence | GAGGCUGGGAUGCCUUUTT | AAAGGCAUCCCAGCCUCCGTT |
| siRNA length base-pair | 19 | 21 |
| siRNA name in paper | M1 | M1 |
| Biological activity | 48 percent target mRNA inhibition | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | Bcl-2 gene | |
| siRNA concentration | 33 nM | |
| Cell or Organism used | HeLa B2 cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 48 Hours | |
| Article title | Asymmetric siRNA targeting the bcl 2 gene inhibits the proliferation of cancer cells in vitro and in vivo | |
| Reference | 23135004 | |