SM2480 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Hydroxyphenyl-Conjugated 0
siRNA modification types 1 0
Overall number of modifications 1 0
Position of modifications 1 0
Modification on sugar or base or phosphate Phosphate 0
SMILES
(Click to view structure & nomenclature)
OC1=CC=C(CCC(=O)NCCCCCCOP(O)(O)=O)C=C1



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siRNA Sequence GGCCUUUCACUACUCCUACGA GUAGGAGUAGUGAAAGGCCAG
siRNA length base-pair 21 21
siRNA name in paper HyPh-siRNA HyPh-siRNA
Biological activity 90 percent target mRNA inhibition
Experiment used to check activity Luciferase reporter assay
Melting temperature (oC) 83.1
Target gene Luciferase gene
siRNA concentration 1 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 48 Hours
Article title SiRNAs conjugated with aromatic compounds induce RISC-mediated antisense strand selection and strong gene-silencing activity
Reference22982308