SM2482 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Pyrene modification 0
siRNA modification types 1 0
Overall number of modifications 1 0
Position of modifications 1 0
Modification on sugar or base or phosphate Sugar 0
SMILES
(Click to view structure & nomenclature)
C1=CC2=C3C(C=CC4=CC=CC(C=C2)=C34)=C



-



siRNA Sequence GGCCUUUCACUACUCCUACGA GUAGGAGUAGUGAAAGGCCAG
siRNA length base-pair 21 21
siRNA name in paper Pyr-siRNA Pyr-siRNA
Biological activity 87 percent target mRNA inhibition
Experiment used to check activity Luciferase reporter assay
Melting temperature (oC) 84.8
Target gene Luciferase gene
siRNA concentration 1 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 48 Hours
Article title SiRNAs conjugated with aromatic compounds induce RISC-mediated antisense strand selection and strong gene-silencing activity
Reference22982308