SM2483 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Locked nucleic acid 0
siRNA modification types 1 0
Overall number of modifications 1 0
Position of modifications 1 0
Modification on sugar or base or phosphate Sugar 0
SMILES
(Click to view structure & nomenclature)
OCC12COC(CO1)C2O



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siRNA Sequence GGCCUUUCACUACUCCUACGA GUAGGAGUAGUGAAAGGCCAG
siRNA length base-pair 21 21
siRNA name in paper siLNA1 siLNA1
Biological activity 86 percent target mRNA inhibition
Experiment used to check activity Luciferase reporter assay
Melting temperature (oC) 84.4
Target gene Luciferase gene
siRNA concentration 1 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 48 Hours
Article title SiRNAs conjugated with aromatic compounds induce RISC-mediated antisense strand selection and strong gene-silencing activity
Reference22982308