Field | Sense | Antisense |
siRNA chemical modification | Locked nucleic acid | 0 |
siRNA modification types | 1 | 0 |
Overall number of modifications | 1 | 0 |
Position of modifications | 1 | 0 |
Modification on sugar or base or phosphate | Sugar | 0 |
SMILES (Click to view structure & nomenclature) |
OCC12COC(CO1)C2O |
- |
siRNA Sequence | GGCCUUUCACUACUCCUACGA | GUAGGAGUAGUGAAAGGCCAG |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | siLNA1 | siLNA1 |
Biological activity | 86 percent target mRNA inhibition | |
Experiment used to check activity | Luciferase reporter assay | |
Melting temperature (oC) | 84.4 | |
Target gene | Luciferase gene | |
siRNA concentration | 1 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 48 Hours | |
Article title | SiRNAs conjugated with aromatic compounds induce RISC-mediated antisense strand selection and strong gene-silencing activity |
|
Reference | 22982308 |