SM2487 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-O-Methyl 2-O-Methyl
siRNA modification types 1 1
Overall number of modifications 8 11
Position of modifications 1,4,8,9,11,14,15,18 1,3,4,7,8,10,13,14,15,17,18
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
COC1C(O)OC(CO)C1O



COC1C(O)OC(CO)C1O



siRNA Sequence CAGCAAACCACGAUCAAUATT UAUUGAUCGUGGUUUGCUGTT
siRNA length base-pair 21 21
siRNA name in paper si-1OMe si-1OMe
Biological activity 5 percent target mRNA inhibition
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene 5'UTR Enterovirus 71
siRNA concentration 50 nM
Cell or Organism used Rhabdomyosarcoma cells
Transfection method Lipofectamine 2000
Duration after transfection 6 Hours
Article title The highly conserved 5' untranslated region as an effective target towards the inhibition of Enterovirus 71 replication by unmodified and appropriate 2'-modified siRNAs
Reference22889374