| Field | Sense | Antisense |
| siRNA chemical modification | 2-Fluoro | 2-Fluoro |
| siRNA modification types | 1 | 1 |
| Overall number of modifications | 8 | 11 |
| Position of modifications | 1,4,8,9,11,14,15,18 | 1,3,4,7,8,10,13,14,15,17,18 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC1OCC(F)C1O |
OCC1OCC(F)C1O |
| siRNA Sequence | CAGCAAACCACGAUCAAUATT | UAUUGAUCGUGGUUUGCUGTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | si-1 F | si-1 F |
| Biological activity | 71 percent target mRNA inhibition | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | 5'UTR Enterovirus 71 | |
| siRNA concentration | 50 nM | |
| Cell or Organism used | Rhabdomyosarcoma cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 6 Hours | |
| Article title | The highly conserved 5' untranslated region as an effective target towards the inhibition of Enterovirus 71 replication by unmodified and appropriate 2'-modified siRNAs |
|
| Reference | 22889374 | |