| Field | Sense | Antisense |
| siRNA chemical modification | 2-O-Methyl | 2-O-Methyl |
| siRNA modification types | 1 | 1 |
| Overall number of modifications | 10 | 8 |
| Position of modifications | 1,6,9,10,12,13,14,16,17,18 | 1,5,9,12,13,15,16,17 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
COC1C(O)OC(CO)C1O |
COC1C(O)OC(CO)C1O |
| siRNA Sequence | CAGAGCAAUUGUUUACCUATT | UAGGUAAACAAUUGCUCUGTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | si -2OMe | si -2OMe |
| Biological activity | 75 percent target mRNA inhibition | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | 5'UTR Enterovirus 71 | |
| siRNA concentration | 50 nM | |
| Cell or Organism used | Rhabdomyosarcoma cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 6 Hours | |
| Article title | The highly conserved 5' untranslated region as an effective target towards the inhibition of Enterovirus 71 replication by unmodified and appropriate 2'-modified siRNAs |
|
| Reference | 22889374 | |