Field | Sense | Antisense |
siRNA chemical modification | 2-Deoxyribonucleotide* Inverted abasic (Mutation) | 0 |
siRNA modification types | 2 | 0 |
Overall number of modifications | 11 | 0 |
Position of modifications | 7,8,9,10,11,12,13,14,15* 1,21 (4,9,13,18) | 0 |
Modification on sugar or base or phosphate | Sugar (Base) | 0 |
SMILES (Click to view structure & nomenclature) |
OCC1OCCC1O CC1COC(CO)C1OP(O)([O-])=O |
- |
siRNA Sequence | CUCAUUTTCTTTGTGCUCACG | CGTTUGCAGAAACAAUUGGUG |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | PTEN GBMM | PTEN GBMM |
Biological activity | Low mRNA target inhibition efficacy | |
Experiment used to check activity | Real-time PCR | |
Melting temperature (oC) | NA | |
Target gene | Human PTEN gene | |
siRNA concentration | 1 to 40 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Oligofectamine (Invitrogen) | |
Duration after transfection | 48 Hours | |
Article title | Structural variations and stabilising modifications of synthetic siRNAs in mammalian cells |
|
Reference | 12771198 |