| Field | Sense | Antisense |
| siRNA chemical modification | 2-Deoxyribonucleotide | 2-Deoxyribonucleotide |
| siRNA modification types | 1 | 1 |
| Overall number of modifications | 2 | 2 |
| Position of modifications | 20,21 | 20,21 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC1OCCC1O |
OCC1OCCC1O |
| siRNA Sequence | CGAGGGGAGUACAUCAAGACC | UCUUGAUGUACUCCCCUCGTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | Akt1 3A | Akt1 3B |
| Biological activity | High target mRNA inhibition efficacy | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | Akt gene | |
| siRNA concentration | NA | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Oligofectamine (Invitrogen) | |
| Duration after transfection | 48 Hours | |
| Article title | Structural variations and stabilising modifications of synthetic siRNAs in mammalian cells |
|
| Reference | 12771196 | |