| Field | Sense | Antisense |
| siRNA chemical modification | 2-Deoxyribonucleotide* Inverted abasic | 2-Deoxyribonucleotide* Inverted abasic |
| siRNA modification types | 2 | 2 |
| Overall number of modifications | 4 | 4 |
| Position of modifications | 22,23 * 1,23 | 22,23 * 1,23 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC1OCCC1O CC1COC(CO)C1OP(O)([O-])=O |
OCC1OCCC1O CC1COC(CO)C1OP(O)([O-])=O |
| siRNA Sequence | CGUUAGCAGAAACAAAAGGAGTT | CUCCUUUUGUUUCUGCUAACGTT |
| siRNA length base-pair | 23 | 23 |
| siRNA name in paper | PTEN5A | PTEN5B |
| Biological activity | Low mRNA target inhibition efficacy | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | Human PTEN gene | |
| siRNA concentration | 1 to 40 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Oligofectamine (Invitrogen) | |
| Duration after transfection | 48 Hours | |
| Article title | Structural variations and stabilising modifications of synthetic siRNAs in mammalian cells |
|
| Reference | 12771196 | |